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91.
Susan Van Noorden Julia M. Polak A. G. E. Pearse 《Histochemistry and cell biology》1977,53(3):243-247
Summary Immunostaining of thin serial paraffin sections has shown that somatostatin is present in the same parafollicular cells as calcitonin in the adult rat thyroid gland. The number of cells containing both peptides is much smaller than the number containing calcitonin but not somatostatin. 相似文献
92.
Isolation of rat liver lysosomes by isopycnic centrifugation in a metrizamide gradient 总被引:9,自引:0,他引:9 下载免费PDF全文
R Wattiaux S Wattiaux-De Coninck M F Ronveaux-dupal F Dubois 《The Journal of cell biology》1978,78(2):349-368
A preparation, similar to the light mitochondrial fraction of rat liver (L fraction of de Duve et al, (1955, Biochem. J. 60: 604-617), was subfractionated by isopycnic centrifugation in a metrizamide gradient and the distribution of several marker enzymes was established. The granules were layered at the top or bottom of the gradient. In both cases, as ascertained by the enzyme distributions, the lysosomes are well separated from the peroxisomes. A good separation from mitochondria is obtained only when the L fraction if set down underneath the gradient. Taking into account the analytical centrifugation results, a procedure was devised to purify lysosomes from several grams of liver by centrifugation of an L fraction in a discontinuous metrizamide gradient. By this method, a fraction containing 10--12% of the whole liver lysosomes can be prepared. As inferred from the relative specific activity of marker enzymes, it can be estimated that lysosomes are purified between 66 and 80 times in this fraction. As ascertained by plasma membrane marker enzyme activity, the main contaminant could be the plasma membrane components. However, cytochemical tests for 5'AMPase and for acid phosphatase suggest that a large part of the plasma membrane marker enzyme activity present in the purified lysosome preparation could be associated with the lysosomal membrane. The procedure for the isolation of rat liver lysosomes described in this paper is compared with the already existing methods. 相似文献
93.
J H Dussault P Walker J D Dubois F Labrie 《Canadian journal of physiology and pharmacology》1977,55(5):1091-1097
94.
Julia F. Morton 《Economic botany》1991,45(3):318-333
The horseradish tree (Moringa pterygosperma,) is being introduced into drought-ridden lands to augment the local food and
fodder supply. The tree grows up to 5 m per year. The foliage is high in calcium and has half the oxalates of amaranth. Seeds
yield edible oil and the seed meal is used as fertilizer and as a coagulant to clarify turbid water. The philanthropic center,
ECHO (Educational Concerns for Hunger Organization), North Fort Myers, Florida, receives many requests for seeds. A missionary
in Mali wrote: “The seeds you sent arrived during the worst year of 14 years of dry weather. Only the moringa survived, and
they have flourished. ”Another seed shipment resulted, after harvesting a crop, in 25 000 trees being planted by university
students and faculty, around laborers’ houses in Maranhao, Brazil. The tree is not limited to tropical lowlands, but thrives
at elevations of 800-1200 m in protected mountain areas of southern Mexico. The long-range effects of ingesting various parts
of the tree as food or folkmedicine need study. Attention should be given to horticultural improvement, perhaps through hybridization
with one or more related species now being compared with M. pterygosperma in India and Africa.
ХРеНовое дерево, Moringa pterygosperma F. Gaertn. (Moringaceae), Дар сыхим землям. Хреновое дерево, Moringa pterygosperma,
вводится в бездождивые землии чтоб умножить местное снабжение пищи и корма. Дерево растет до 5 метров в год. Листья содержат
много кальцию и половину щавелев по сравнени#x044E; с амарантом. Семя дают съедобное масло и семеная мука употребляется как
одобрение и как коагулант для очищение мутной воды. Филантропический цэнтэр ECHO (Educational Concern for Hunger Organization,
North Fort Myers, Florida) полужает мното просьб чтоб получить семя. Один миссионер из Мали писал “Семя которые вы послали,
прибыли в самый сухой год за 14 лет сухой погоды. Только моринга пережила и цвела. Другая отправка семен была совершена после
получения урожая и 25000 деревьев были посажены студентами и учителями около домов рабочих в Маранхау, Брразилия.
Это дерево не ограаничевается тропическим климатом и преуспевает на уровне 800 до 1200 метров в защищенных горных местах южной
Мексики. 相似文献
95.
Summary The use of conjugates of microbial iron chelators (siderophores) and antibiotics for illicit transport of antibiotics into cells is a potentially powerful method for the rational design of therapeutic agents. The structural complexity of most natural siderophores has impeded progress in this area. Described here are the design, syntheses and preliminary biological studies of several siderophore--lactam antibiotic conjugates. Both hydroxamic-acid-based and catechol-based conjugates with and without amino acid spacers to carbacephalosporins were synthesized and demonstrated to be effective inhibitors ofEscherichia coli X580. Mutant selection was noted for each class of conjugates. Mutants selected from exposure of theE. coli to the hydroxamate conjugates were susceptible to the catechol conjugates and vice versa. Combinations of hydroxamate-and catechol-carbacephalosporin conjugates were most effective inhibitors ofE. coli X580. 相似文献
96.
1-Tributylstannyl-D-glucals, prepared from the corresponding 1-phenylsulfonyl-D-glucals, were coupled efficiently to various organic halides in the presence of a palladium(0) catalyst. This mild reaction is specially useful for the preparation of 1-C-aryl-D-glucals and compatible with unprotected hydroxy groups or hindered aromatic bromides. It has been shown that the resulting 1-C-aryl(alkyl)-D-glucals are suited for further synthetic manipulation of the enol ether group, including stereoselective hydrogenation, hydroboration-oxidation, or epoxidation. All compounds formed resulted from the attack of the alpha-face of the glucal derivatives by the reagent. The reaction, extended to 1,3-, 1,4-di-, and 1,3,5-tri-bromobenzenes, leads to the corresponding symmetrical di-(tri)-C-glucosylbenzenes. Finally, a sequential di-C-glucosylation of 1,3-dibromobenzene with two different 1-stannylated glucals was obtained. 相似文献
97.
Nuñas (Thaseolus vulgaris, Fabaceae), commonly called popping beans, are traditionally grown in the Andean highlands of South America, and are consumed as a snack food after a quick toasting process. Proximate analysis of their nutritive value revealed that nunas have a higher content of starch, amylose, and copper than four dry bean varieties and a lower mean content of protein, phosphorous, iron, and boron. The unique texture and taste of nuñas may be related to their high starch content. Antinutritional factors such as lectins were higher in raw and boiled nuña samples than in toasted nuñas, while tannin levels did not change from raw to toasted treatments. Overall in-vitro digestibility was slightly lower for toasted nunas than boiled dry bean. 相似文献
98.
Purification and immunocytochemical localization of kafirins inSorghum bicolor (L. Moench) endosperm
Summary Kafirins are the storage proteins of sorghum and are found in protein bodies in the seed endosperm. They have been classified as -, -, and -kafirins according to differences in molecular weight, solubility, and structure. The kafirins were purified, amino acid composition was determined, and immunolocalization methods were used to determine the organization of the protein bodies and distribution of kafirins throughout the endosperm. All three groups of kafirins were low in lysine. -Kafirins and -kafirins were relatively high in cysteine, and -kafirins were relatively high in methionine. Transmission electron microscopy showed that protein bodies in the peripheral endosperm were spheroid with concentric rings and few darkly stained inclusions. In contrast, protein bodies of the central endosperm were irregularly shaped with a higher proportion of darkly stained material. The light staining regions of the protein bodies are composed primarily of -kafirins with minor portions of - and -kafirins. The dark staining regions, however, are composed primarily of - and -kafirins. Immunoelectron microscopy showed that protein bodies in the peripheral endosperm contain predominantly a-kafirin with minor amounts of - and -kafirin. Central endosperm protein bodies are also predominantly -kafirin, but have a higher proportion of -kafirin and -kafirin than the peripheral endosperm protein bodies.Abbreviations GAR-HRP
Goat anti-rabbit horseradish peroxidase
- IgG
immunoglobulin G
- 2-ME
2-mercaptoethanol
- SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis
- TBS
Tris buffer saline
- TBS-T
Tris buffer saline with Tween
- TBS-T-B
Tris buffer saline with Tween and bovine serum albumin
- TCA
trichloroacetic acid
- UV
ultraviolet 相似文献
99.
Neuropeptide Y modulates the action of vasodilator agents in guinea-pig epicardial coronary arteries
S rgio Gulbenkian Lars Edvinsson Ole Saetrum Opgaard Angela Valen a John Wharton Julia M. Polak 《Regulatory peptides》1992,40(3):351-362
Recently, we have demonstrated that guinea-pig epicardial coronary arteries are supplied by numerous nerve fibres containing neuropeptide Y (NPY) immunoreactivity. However, examination of vasomotor responses revealed that NPY did not elicit a contractile response in these arteries. In contrast, acetylcholine (ACh), calcitonin gene-related peptide (CGRP), substance P and vasoactive intestinal polypeptide (VIP) all relaxed precontracted arteries. In the present study, we have used histochemical, immunohistochemical and in vitro pharmacological techniques, in order to further investigate the possible role of NPY in guinea-pig epicardial coronary arteries. A double-immunofluorescence staining technique revealed that CGRP and substance P were co-localized in nerve fibres distinct from those displaying NPY immunoreactivity. Furthermore, using a method combining immunofluorescence and histochemical techniques, we observed that putative cholinergic nerve fibres (identified by their acetylcholinesterase content) and NPY-immunoreactive nerve fibres are two different nerve populations. An in vitro pharmacological method demonstrated that NPY markedly inhibited the relaxant responses mediated by ACh, VIP, substance P and isoprenaline but had no effect on CGRP. These results suggest that NPY-containing nerves associated with guinea-pig epicardial coronary arteries may be predominantly involved in modulating the action of vasodilator agents. 相似文献
100.
J Dubois C Dugave C Fourès M Kaminsky J C Tabet S Bory M Gaudry A Marquet 《Biochemistry》1991,30(43):10506-10512
The stereochemical course of the vitamin K dependent carboxylation has been elucidated using a (4S)-4-fluoroglutamyl-containing pentapeptide as a substrate. The absolute configuration of the [13C]-4-carboxy-4-fluoroglutamate obtained when the carboxylation was carried out with 13C-labeled sodium bicarbonate, was determined after reduction of the [13C]-4-carboxy-4-fluoroglutamyl residue into 4-fluoro-5,5'-dihydroxyleucine, hydrolysis, lactonization, and peracetylation. The absolute configuration at C-4 was determined to be S by locating the 13C label in the lactone ring of the trans isomeric lactone and in the hydroxymethyl group of the cis isomer following HPLC separation of both isomers and analysis by GC/MS/MS techniques. It follows that the vitamin K dependent carboxylation occurs with inversion of configuration. 相似文献